GLUT 1 (Glucose Transporter 1)

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.\nGLUT 1 is a facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. It has a very broad substrate specificity and can transport a wide range of aldoses including pentoses and hexoses. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:50,000; Control peptide can be used to coat ELISA plates at 1ug/ml.\nWestern Blot: 1:1000-1:5000; Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes (1). Not recommended to heat the samples prior to loading to prevent Glut protein aggregation.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide: \nG3900-01D: GLUT 1, Mouse, Control Peptide (Glucose Transporter 1)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3900-01A

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Prc, Rabbit

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Haspel, et al., J. Biol. Chem. 263: 398-403 (1986). 2.

Additional Info

Recognizes mouse Glut-1. No significant sequence homology with other Glut proteins. Species Sequence Homology: 100% conserved in human, rat, rabbit, bovine and porcine; 90% conserved in chicken.