GLUT 2 (Glucose Transporter, Insulin Regulatable)

GLUT 2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. GLUT 2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 2.5ug to label 1x10e6 NTERA-2 human human cell line\nImmunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (medulla), liver, normal pancreas array and pancreatic cancer tissue \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3900-15N

Size

100ug

Applications

FC, ICC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 5% trehalose.

P Type

Mab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG2a

References

1. Olson, A.L. and Pessin, J.E., Annu. Rev. Nut. 16: 235-256 (1996). 2. Mahraoui, L., et al., J. Biochem. 298(3): 629-633 (1994).

Additional Info

Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on hGLUT 2-transfected NS0 cells, but not on control transfectants. Detects GLUT 2 on the surface of the human intestinal cell line Caco-2 in Flow Cytometry and on fixed cells in Immunocytochemistry. Based on Flow Cytometric tests on transfected cells, does not crossreact with human GLUT 1 or GLUT 3.