GLUT 2, Rat (Glucose Transporter, Insulin Regulatable) (Control Peptide)

Control Peptide for G3900-15D (affinity purified antibody) and G3900-15 (antiserum). G3900-15F is 16 amino acid peptide sequence corresponding to the exoplasmic loop between helices 1 and 2 of rat liver Glucose Transporter. Rat GT21 peptide sequence has 93% identity with mouse and human Glut-2 (15/16 AA).\n\n\nMost mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, 40-60kD) and sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, throughout the blood brain barrier, and to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons. Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa, , monkey 621aa;~ 50 kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue and small intestine. Glut-13 or proton myo-inositol transporter (HMIT; human 629aa, rat 618aa, ~75-90 kD/67 kD protein) is highly expressed in glial cells and some neurons. Glut-13 transport activity was specific for myo-inositol. Rat HMIT is ~35% identical to rat GlutX1.\n\nApplications: \nSuitable for use in ELISA, Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml.\nAntibody Blocking: 5-10ug per 1ug G3900-15D (affinity purified antibody) or per 1ul G3900-15 (antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts