GLUT 5 (Glucose Transporter)

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-60kD) and Sodium glucose co-trasporter-1 (SGLT-1, 662aa, ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL. The antibody has been shown to react with a protein band of about 60kD in the rat jejunum membranes that is slightly larger than that reported in human GLUT 5 (50-55kD). In rat kidney, a protein of ~42kD was detected. \nELISA: 1:1000- 1:10000. Use G3900-65G control peptide to coat ELISA plates at 1ug/ml.\nImmunohistochemistry: See reference (2).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3900-65D

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Garcia MDL (2003)J, . Neurochem., Aug 2003; 86: 709 - 724. WB; 2. Garcia MDL (2003)J, . Neurochemistry 86 3, 709-724. IHC. 3. Venge P, 2003, Respiratory Med. 97, 1109-119. 4. Shu h-J, 2006, Neuroscience in press. IF

Additional Info

Recognizes rat Glut-5. Species sequence homology: human: 61%, mouse: 53%.