GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1- Glut-14, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis.\n\nApplications:\nSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-5ug/ml (ECL). Do not to heat the samples prior to loading to prevent gluts aggregation. Mouse tissues or mouse insulinoma beta TC-6 cells can be used as positive control.\nFlow Cytometry: ~10ug/ml (10e5 cells) Perform the antibody binding in small volume of ~200ul.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3900-15Q

Size

100ug

Applications

FC, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS. Reconstitute with 100ul of sterile PBS.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography

Isotype

IgG2b

References

1. Fukumoto, H., et al (1988) Proc. Natl. Acad. Sci. 85, 5434-5438. 2. Thorens, B, et al (1988) Cell, 55, 281- 290. 3. Asano, T et al (1989) Nucleic Acid Res. 17, 6386. 4. Suzue, K., et al (1989) Nucleic Acid Res. 17, 10099.

Additional Info

Recognizes mouse GLUT2.