Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-3 is detectable in normal cell type of: spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT-3 staining is detected in human cancers of the ovary, lung, and testis.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5ug/ml for 2h
Immunohistochemistry: 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:
Placenta
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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