Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-4 (mouse/human 509aa) is primarily expressed in muscle and adipose tissue and regulated by insulin.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1-4ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
3T3 cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.