Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoform differ in their tissue expression, substrate specificity and kinetic characteristics.
Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions:
Western Blot: 1-5ug/ml (ECL)
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:
G3900-80J: GLUT7, Control Peptide (Solute Carrier Family 2, Facilitated Glucose Transporter Member 7, Glucose Transporter Type 7, GLUT-7, SLC2A7)
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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