Glutamate/Aspartate Transporter 2 (GLT1, EAAT2, Excitatory Amino Acid Transporter 2)

The activity of Glutamate/Aspartate Transporter 2 (also known as GLT1), the main glutamate transporter in the CNS, can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the Glutamate/Aspartate Transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the Glutamate/Aspartate Transporter 2. Exposure to PMA increases the ubiquitylation of the Glutamate/Aspartate Transporter 2 in transfected cells and in the rat brain, and this ubiquitylated Glutamate/Aspartate Transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Glutamate/Aspartate Transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml detected Glutamate/Aspartate Transporter 2 on 10ug of mouse brain tissue lysate.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse brain tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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