Glutamate Decarboxylase 65 (GAD65, DCE 2, DCE2, GAD 2, GAD 65 GAD-2, GAD-65, GAD2, Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa), Glutamate Decarboxylase 2, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65)

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.\n\nCellular Localization: Cytoplasmic\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4000-09

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Meyre D, et al. Is Glutamate Decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children? J Clin Endocrinol Metab. (2005)[Epub ahead of print] PMID: 15671113

Additional Info

Species Crossreactivity: Crossreacts with Human. Expected to react with mouse, rat, canine and chimpanzee based on sequence similarity.

Alternative Names

GAD65, DCE 2, DCE2, GAD 2, GAD 65 GAD-2, GAD-65, GAD2, Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa), Glutamate Decarboxylase 2, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65