Glutamate Decarboxylase 65 (GAD65, DCE 2, DCE2, GAD 2, GAD 65 GAD-2, GAD-65, GAD2, Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa), Glutamate Decarboxylase 2, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65)

Glutamate decarboxylase (GAD) catalyzes the production of gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter. GAD is represented by two isoforms, GAD1 and GAD2, which are the products of two different genes and differ substantially only at their N-terminal regions. In neurons and beta cells, GAD is concentrated around synaptic vesicles and synaptic-like microvesicles, respectively, as well as in the area of the Golgi complex. GAD2 lies primarily in axon terminals and only about half of GAD2 exists as active holoenzyme (1-2). The beta cell-specific marker GAD2 can be quantified in pancreatic cell extracts and in serum (3). GAD2 is a major target for autoantibodies and autoreactive T cells in patients with insulin-dependent diabetes mellitus. Autoantibodies to GAD are also found in patients with stiff man syndrome or polyendocrine autoimmunity (4).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n \nRecommended Dilution:\nWestern Blot: 1:500-2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

G4000-09C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. M Solimena, et al. PNAS 90(7):3073-3077, 1993 2. Kaufman DL, et al. J Neurochem. 56(2):720-3, 1991 3. Rui M, et al. J Immunol Methods 19(1-2):133-43, 2007 4. Davenport C, et al. Clin Exp Immunol. 111(3):497-505,1998

Additional Info

Recognizes human GAD2.