Gamma-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain. Glutamic acid decarboxylase (GAD; E.C.4.1.1.15) catalyzes the decarboxylation of glutamate to produce GABA-the rate limiting step in the synthesis of GABA. The two major isoform of GAD, GAD67 (MW 67kD) and GAD65 (MW 65kD) are derived from distinct genes and differ in their subcellular distribution. GAD67 is 594 aa single chain polypeptide encoded by human chromosome 2 gene, whereas GAD65 is an amphiphilic and membrane anchored protein (585 aa, chromosome 10). GAD isoform shares ~65% homology. GAD is also a major autoantigen associated with SMS (stiff-man syndrome).
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10 ug/ml using Chemiluminescence technique
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide: G4000-01A: Glutamate Decarboxylase 65, Rat, Control Peptide (GAD65)
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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