$Glutamate Decarboxylase 65, Rat, Control Peptide\n(GAD65)$

Supplier:

United States Biological

Cat no: G4000-01A

Glutamate Decarboxylase 65, Rat, Control Peptide\n(GAD65)

Control Peptide for G4000-01G (ascites) and G4000-01D (affinity purified)\n\nAn 18-aa peptide sequence near the amino terminus of rat GAD65 .\nGamma-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain. Glutamic acid decarboxylase (GAD; E.C.4.1.1.15) catalyzes the decarboxylation of glutamate to produce GABA-the rate limiting step in the synthesis of GABA. The two major isoform of GAD, GAD67 (MW 67kD) and GAD65 (MW 65kD) are derived from distinct genes and differ in their subcellular distribution. GAD67 is 594 aa single chain polypeptide encoded by human chromosome 2 gene, whereas GAD65 is am amphiphilic and membrane anchored protein (585 aa, chromosome 10). GAD isoform shares ~65% homology. GAD is also a major autoantigen associated with SMS (stiff-man syndrome).\n\nApplications:\nSuitable for use in ELISA. Not suitable for Western Blot, low MW:<3kD, Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 5-10ug control peptide/1ug of affinity pure IgG or 1ul antiserum.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4000-01A

Size

50ug

Applications

ELISA

Reactivities

Rat, Prc

Form

Supplied as a liquid in PBS, pH 7.5.\n

Purity

Highly purified

References

1. Erlanger MG et al (1991) Neuron 7, 91-100. 2. Dirkx R et al (1995) J Biol. Chem. 270, 2241

Additional Info

Species sequence homology: Porcine: 100%, mouse-94%, human and monkey-83%.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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