Glutamate Decarboxylase 67 (GAD67)

Glutamic acid decarboxylase (GAD) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid\n(GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% amino acid identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. GAD67 is cytoplasmic (594aa), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation or disease progression.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5000-1:10,000; Using ECL on rat and mouse brain lysate.\nImmunohistochemistry (Paraffin): 1:500; No epitope retrieval necessary.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSKNSH cell lysate (human neuroblastoma), mouse cerebral cortex\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G4000-20

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Ladewig, J., et al., Stem Cells 26(7): 1705-1712 (2008). 2. King, A.E., et al., J. Comp. Neurol. 498: 277-294 (2006). 3. Ling, L.L., et al., Neuroscience 132: 1103-1113 (2005). 4. Watanabe, K., et al., Nature Neurosci. 8: 288-296 (2005). 5. Varea, E., et al., Neuroscience 136: 435-443 (2005).

Additional Info

Recognizes rat Glutamate Decarboxylase 67 at ~67kD. No detectable crossreactivity with GAD65 by Western Blot on rat brain lysate. Species Crossreactivity: human and mouse.