Glutamate Dehydrogenase (GDH, GLDH, GLUD 1, GLUD, GLUD1, GLUD1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 1 mitochondrial, MGC132003)

Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase GDH1, that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix.\n\nApplications: \nSuitable for Immunoblotting (western or dot blot), ELISA, and conjugation. Other applications have not been tested.\n\nRecommended Dilution: \nELISA: 1:5000 -1:20,000\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4000-51

Size

10mg

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Bov

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.

P Type

Pab

Purity

Puri?ed by delipidation, fractionation and ion exchange chromatography.

Isotype

IgG

Additional Info

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified bovine liver glutamate dehydrogenase.