Glutamate (Inhibitory Protein Factor, IPF)

Inhibitory protein factor (IPF) refers to a three distinct proteins with ~mol wt of 138kDa (IPF-alpha), 135kD (IPF-beta), and 132kD (IPF-gamma). IPF-a is derived from a ubiquitous, non-erythroid brain spectrin called alpha-Fodrin, a well-characterized protein previously implicated in such diverse activities as exocytosis/endocytosis, apoptosis, and NMDA-receptor activation. However, a-Fodrin itself has no effect on glutamate uptake. The N-terminal 1-20 aa of IPF-a, IPF-b, and IPF-g are identical with 26-45 aa of a-Fodrin (mol wt ~240kD). Therefore, it appears that some identified protease(s) may generate IPF-a from a-Fodrin. IPF does not inhibit ATP-independent uptake, norepinephrine uptake into chromaffin vesicles, and Na-dependent glutamate uptake into synaptosomes.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL technique. \nELISA: 0.5-1ug/ml. Coat plates with 1ug/ml control peptide. \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: G4000-72\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4000-71

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

(1) Ozkan ED et al (1997) PNAS 94, 4317-4142; Tamura Y et al (2001) J. Neurochem. 76, 1153-1164; moon RT et al (1990) JBC 265, 4427; McMahon AP et al (1987) Biochem. Soc,. Trans. 15, 804; Hong WJ et al (1989) JBC 264, 12758.

Additional Info

Recognizes human IPF. Species Sequence Homology: Rat:100%; Chicken spectrin alpha chain: 90%.\n\n\n