Glutamate Pyruvate Transaminase, Recombinant, Human, aa1-496, His-tag (Alanine Aminotransferase 1, ALT1, Glutamate Pyruvate Transaminase 1, GPT 1, Glutamic-alanine Transaminase 1, Glutamic-pyruvic Transaminase 1, AAT1, GPT1, GPT)

Supplier:

United States Biological

Cat no: G4010-03

Glutamate Pyruvate Transaminase, Recombinant, Human, aa1-496, His-tag (Alanine Aminotransferase 1, ALT1, Glutamate Pyruvate Transaminase 1, GPT 1, Glutamic-alanine Transaminase 1, Glutamic-pyruvic Transaminase 1, AAT1, GPT1, GPT)

GPT, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and aa. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings.\n\nSource:\nRecombinant corresponding to aa1-496 from human GPT, fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS \n\nMolecular Weight:\n~56.8kD (MALDI-TOF)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4010-03

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 20% glycerol, 2mM DTT.

Purity

~90% (SDS-PAGE)

References

1. Sohocki M.M. et al. (1997) Genomics 40: 247-252. 2. Matthews C.C. et al. (2003) Brain Res. 978: 59-64.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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