Glutamate Transporter 1b EAAT2b (GLT1b, GLT1v, Slc1a2, Solute Carrier Family 1, Glial High Affinity Glutamate Transporter Member 2)

GLT1 is the major glial glutamate transporter that takes up more than 90% of glutamate from the synapse. GLT1b is a splice variant that contains 11 unique amino acids at the C-terminus that encode a PDZ binding domain. GLT1b was initially believed to be the neuronal isoform of GLT1, but it now appears that GLT1b is localized to astrocytes in the normal brain and to neurons in pathological conditions. In Lou Gehrig's Disease, GLT1a is decreased, while GLT1b is increased. There are also different expressions in hypoxic cells, which have not yet been fully elucidated.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:200-1:500. A band is seen at ~70kD, representing the GLT1b protein. A band may also be observed at ~50kD.\nImmunohistochemistry: 1:200-1:500\nImmunocytochemistry: 1:200-1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTransfected COS7 lysate and rat brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G4003-91B

Size

100ul

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2. No preservatives added.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1. Lauriat, T.L. & McInnes, L.A., Mol. Psychiatry 12: 1065-1078 (2007). 2. Berger, U.V., et al., J. Comp. Neurol. 492(1): 78-89 (2005). 3. Yi, J.H., et al., Glia 49(1): 121-133 (2005). 4. Pow, D.V., et al., Brain Res. Dev. Brain Res. 153(1): 1-11(2004). 5. Maragakis, N.J., et al., Ann. Neurol. 55(4): 469-77 (2004). 6. Chen, W., et al., J. Neurosci. 22(6): 2142-2152 (2002). 7. Schmitt, A., et al., Neuroscience 109(1): 45-61 (2002).

Additional Info

Recognizes rat GLT1b. Does not react with GLT1a. \nSpecies Crossreactivity: Mouse. Does not react with human.

Alternative Names

GLT1v, EAAT2b, Glutamate Transporter 1b, Slc1a2, Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2