Glutamate Transporter, Aspartate EAAT1 (GLAST)

Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4 and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display ~55% homology and are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and Purkinje cells, and specifically enriched in cortex, hippocampus, and caudate-putamen and confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina.\nGLAST (sodium dependent Glutamate/Aspartate Transporter 1, or Excitatory Amino Acid Transporter 1, EAAT1) is a glutamate transporter. It is a transmembrane protein with ~543 amino acids and a molecular weight of 67kD.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml (ECL) \nELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See G4003-90A.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G4003-90

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tanaka, K., (1993) Neurosci. Lett. 159: 183. 2. Storck, T., et al., (1992) PNAS 89: 10,955. 3. Tanaka, K., (1993) Neurosci. Lett. 16: 149. 4. Shashidharan, P., et al., (1993) BBA 1216: 161. 5. Rothstein, J.D., et al., (1994) Neuron 13: 713. 6. Rothstein, J.D., et al., (1995) Ann. Neurol. 38: 73-84. 7. Alexander, G.M., et al., (2000) J. Neurochem. 74: 1,666. 8. Ueda, Y., et al., (2000) Exp. Brain Res. 133: 334-340. 9. Ueda, Y., et al., (2000) Epilepsy Res. 39: 201. 10. Ueda, Y., et al., (2001) J. Neurochem. 76: 892. 11. Deitch, J.S., et al., (2002) J. Neurosci. 193: 117.

Additional Info

Recognizes rat GLAST.