Glutamate Transporter, Aspartate EAAT1 (GLAST)

Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4 and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display about 55% homology. They are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and Purkinje cells. It is specifically enriched in cortex, hippocampus and caudate-putamen. It is confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina. GLAST (sodium dependent Glutamate/Aspartate Transporter 1, or Excitatory Amino Acid Transporter 1, EAAT1) is a glutamate transporter. It is a transmembrane protein with ~543aa and a molecular weight of 67kD.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5,000 \nELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 5-10ug/1ul serum.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nG4003-90A: Glutamate Transporter, Aspartate EAAT1, Rat (GLAST)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G4003-90B

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Tanaka, K., Neurosci. Lett. 159: 183 (1993). 2. Storck, T., et al., PNAS 89: 10,955. (1992) 3. Tanaka, K., Neurosci. Lett. 16: 149 (1993). 4. Shashidharan, P., et al., BBA 1216: 161 (1993). 5. Rothstein, J.D., et al., Neuron 13: 713 (1994). 6. Rothstein, J.D., et al., Ann. Neurol. 38: 73-84 (1995). 7. Alexander, G.M., et al., J. Neurochem. 74: 1666 (2000). 8. Ueda, Y., et al., Exp. Brain Res. 133: 334-340. (2000) 9. Ueda, Y., et al., Epilepsy Res. 39: 201 (2000). 10. Ueda, Y., et al., J. Neurochem. 76: 892 (2001). 11. Deitch, J.S., et al., J. Neurosci. 193: 117 (2002).

Additional Info

Recognizes rat GLAST. No significant sequence homology to other glutamate transporters. Species Sequence Homology: Mouse, human and bovine: 100%; chicken and canine: 93%.