Glutamate Transporter EAAC1

L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identi?ed: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters (2) are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene (3) develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml \nWestern Blot: 1-5ug/ml \nImmunoprecipitation: 1-5ug/IP reaction \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4005-50A

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Shashidharan, P, et al., Immunohistochemical localization of the neuron-specific glutamate transporter EAAC1 (EAAT3) in rat brain and\nspinal cord revealed by a novel monoclonal antibody. Brain Research 773:139, (1997).\n2. Slotboom DJ, et al., Structural features of the glutamate transporter family. Microbiol Mol Biol Rev. 63(2):293-307 (1999).\n3. Peghini P, et al., EMBO J., Glutamate transporter EAAC-1-deficient mice develop dicarboxylic aminoaciduria and behavioral\nabnormalities but no neurodegeneration.

Additional Info

Recognizes the ~70kD glutamate transporter EAAC1. Species Crossreactivity: rat and human glutamate transporter EAAC1.