Glutamate Transporter EAAT2, Control Peptide, Rat (GLT1)

Control Peptide for G4005-91 (affinity purified) and G4005-91B (antiserum).\n\nGlutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4 and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display about 55% homology and are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and Purkinje cells. It is specifically enriched in cortex, hippocampus and caudate-putamen. it is confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommendedDilution:\nELISA: Coat plate at 1ug/ml.\nAntibody Blocking: 5-10ug per 1ug affinity pure IgG (G4005-91) or 1ul antiserum (G4005-91B).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G4005-91A

Size

50ug

Applications

ELISA

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

Purity

Highly purified

References

1. Pines, G., et al. (1992) Nature 360: 464-467. 2. Kanner, B., et al. (1993) FEBS Lett. 325: 95-99. 3. Zhang, Y., et al. (1994) J. Biol. Chem. 269: 19573-19577. 4. Grunewald, M. and Kanner, B. (1995) J. Biol. Chem. 270: 17017-17024. 5. Arriza, J.L., et al. (1994) J. Neurosci. 14: 5559-5569. 6. Krischner, M.A., et al. (1994) Genomics 24: 218-224. 7. Zhang, Y., et al. (1994) J. Biol. Chem. 269: 19573-19577. 8. Grunewald, M. and Kanner, B. (1995) J. Biol. Chem. 270: 17017-17024.

Additional Info

Synthetic peptide (16aa) from the cytoplasmic, N-terminus of rat GLT1. Has no significant sequence homology to other glutamate transporters. Species Sequence Homology: Mouse: 100%; Human: 81%.