Glutamate Transporter, Glial (GLAST, EAAT1, GLT-1)

Glutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented. There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2. mRNA has been identified in brain for each of these proteins.\n\nEAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.\n\nApplications:\nSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nImmunohistochemistry (Paraffin): 1:1000 using heat-induced epitope retrieval (HIER).\nWestern Blot: 1:500 detected GLT-1 on 10ug of mouse brain lysates\nOptimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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