Glutamate Transporter, Neuronal (EAAC1, EAAT3)

The antibody has been tested on central nervous system tissue. The staining pattern for the cell bodies obtained corresponds to the pattern described using in situ hybridization with probes to rEAAC1 mRNA. The immunoreactivity corresponds to what has been described for glutamatergic neuronal systems. Preadsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining\n\nApplications:\nSuitable for use in Immunohistochemistry. Not recommended for use in Western blot. Other applications not tested. \n\nRecommended Dilutions: \nImmunohistochemistry: 1:4000-1:8000 using a cyanine-conjugated secondary antibody. Enzymatic methods of detections can be used, however antibody dilution must be increased to 1:10,000-1:30,000. Lightly fixed, 4% PFA material and the use of triton X-100 in the block only is recommended. \nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G4005-40

Size

50ul

Applications

IHC

Hosts

Goat

Reactivities

Mouse, Rat, GP

Form

Supplied as a liquid. No preservatives added.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Nature (1992) 360:467

Additional Info

Recognizes rat Neuronal Glutamate Transporter (EAAC1; also called EAAT3). Species Crossreactivity: mouse and guinea pig. Species sequence homology: human, rabbit and monkey.