Glutamic Acid Decarboxylase 67 (Glutamate Decarboxylase 67 kD Isoform, GAD67, GAD-67, 67 kD Glutamic Acid Decarboxylase, GAD, Glutamate Decarboxylase 1, GAD1, FLJ45882, SCP)

Glutamate Decarboxylase (EC 4.1.1.15) catalyzes the conversion of L-glutamic acid to GABA, a major inhibitory neurotransmitter in the CNS. GAD67, a 67kD protein, is a neuronal isoform of this enzyme predominantly expressed in human Brain. It consists of Pyridoxal 5'-phosphate binding site and conserved Cysteine residues which are vital for its activity and is inhibited due to phosphorylation. It has high binding affinity for the cofactor Pyridoxal-5'phosphate and its role has been implicated in normal Palate development. It is found to be associated with childhood onset schizophrenia and craniofacial malformations and has been identified as a major auto antigen and an auto-reactive T-cell target in IDDM. Deficiency of this enzyme leads to pyridoxine dependency along with epilepsy. It also might play a role in stiff main syndrome.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSK-N-SH\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G4000-12K

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Asada et.al PNAS 94: 6496-6499 (1997) 2. Wei et.al J Neurochem., 93(3):624-33 (2005 May) 3. Addington et.al Mol Psychiatry., 10(6):581-8 (2005 Jun) 4. Steven et.al J Biol Chem, 275 (7); 5188-5192 (February 18, 2000).

Additional Info

Recognizes human GAD67. Species sequence homology: mouse and rat.

Alternative Names

Glutamate Decarboxylase 1 (Brain, 67kDa), EC=4.1.1.15