Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66.9kD protein (594aa) selectively expressed in gabanergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter b-amino butyric acid (GABA).
An affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (CAA01912) and human (NP_000808) gene products.
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions:
Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells
Western Blot: 1:2000-1:5000
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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