Glutamine Synthetase (GS, Glutamate Decarboxylase, Glutamate-ammonia Ligase, GLUL, GLNS)

Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. This reaction consumes a molecule of ATP:Glutamate + NH4+ + ATP , and produces, Glutamine + ADP + Pi. It is found in astrocytes as an octamer of identical 45kD subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in controlling metabolic regulations of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. On the other hands, the level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.\n\nApplications: \nSuitable for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: 1-2ul/400ul lysates\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts