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Glutaredoxin-2, aa20-164, Recombinant Human, His-Tag (GRX2)

Cat no: G5012

Glutaredoxin-2, aa20-164, Recombinant Human, His-Tag (GRX2)

Glutaredoxin-2, as known as GRX2, multifunctional enzyme with glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase(GST) activity. This protein is localized both in the mitochondria and nucleus. It has anti-apoptotic activity by preventing cardiolipin oxidation and cytochrome c release. GRX2 exhibited 36% identity with GRX1 and had a disulfide active center containing the Cys-Ser-Tyr-Cys motif. Recombinant human Glutaredoxin 2, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.\n\nSource:\nRecombinant corresponding to human Glutaredoxin 2, his-tagged, expressed in E. coli.\n\nAA sequence:\nMSAGWLDRAA GAAGAAAAAA SGMESNTSSS LENLATAPVN QIQETISDNC VVIFSKTSCS YCTMAKKLFH DMNVNYKVVE LDLLEYGNQF QDALYKMTGE RTVPRIFVNG TFIGGATDTH RLHKEGKLLP LVHQCYLKKS KRKEFQLEHH HHHH\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

G5012

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1 mM PMSF, 10% glycerol.

Purity

~90% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.Elliott SJ., et al. (2009) Biochemistry. 948(18):3813-5.\n2.Das DK., et al. (2008) J Mol Cell Cardiol. 44(2):252-60

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