The B-Bridge Low Volume Serum Creatinine kit is designed to quantitatively measure creatinine present in serum samples using a 384-well plate format to minimize sample usage. Only 15 uL of serum or plasma is needed per well. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve. Standards or samples are pipetted into the clear 384 well microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm in 384-well plates. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards. A free Excel worksheet available for download at our web site.
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