Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. Kit provides a simple in vitro assay for detecting total glutathione changes during apoptosis and other conditions. The assay utilizes monochlorobimane (MCB), a dye that forms an adduct with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex/Em = 380/461 nm) when bound to glutathione. The reaction is catalyzed by glutathione S- transferase. The assay detects both reduced and oxidized glutathione.
Kit Components:
G8124A: Cell Lysis Buffer 1x25ml
G8124B; Monochlorobimane 1x200ul
G8124C: GST Reagent (50u/ml) 1x200ul
G8124D: GSH Standard (1 mg; MW: 307) 1x1vial
Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -20 degrees C. Store G8124A at 4 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
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