Glutathione GSH/GSSG Detection BioAssay(TM) Kit (Cuvette)

United States Biological has improved the standard GSH/GSSG Assay Kit by using a new and improved GSH scavenger.\n\nReduced glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) that contains a free thiol group. GSH is a major tissue antioxidant that provides reducing equivalents for the glutathione peroxidase (GPx) catalyzed reduction of lipid hydroperoxides to their corresponding alcohols and hydrogen peroxide to water:\n\nH2O2 + 2GSH ---------> GSSG + 2H2O (catalyzed by GPx)\n\nIn the GPx catalyzed reaction, the formation of a disulfide bond between two GSH molecules gives rise to oxidized glutathione (GSSG). The enzyme glutathione reductase (GR) recycles GSSG to GSH with the simultaneous oxidation of beta-nicotinamide adenine dinucleotide phosphate (beta-NADPH):\n\nGSSG + NADPH -----------> 2GSH + NADP+ (catalyzed by GR)\n\nWhen cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease. Therefore, the determination of the GSH/GSSG ratio and the quantification of GSSG are useful indicators of oxidative stress in cells and tissues.\n\nKit Components:\nG8124-11A: Assay Buffer, 5X, 1x50ml. General buffer used to dilute samples and reagents\n*G8124-11B: GSSG Standard (10uM), 1x2ml.\nG8124-11C: Scavenger, 1x3ml. Thiol scavenger to keep GSSG in oxidized form.\nG8124-11D: DTNB, 4x1vial. Lyophilized 5,5'-dithiobis-2-nitrobenzoic acid.\n*G8124-11E: MPA, 5%, 1x40ml. Metaphosphoric acid used to deproteinate samples.\n*G8124-11F: Glutathione reductase, 1x130ul. Solution containing recombinant glutathione reductase.\nG8124-11G: NADPH, 4x1vial. Lyophilized beta-nicotinamide adenine dinucleotide phosphate.\n\nStorage and Stability:\nStore *G8124-11B, *G8124-11E and *G8124-11F at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8124-11

Size

1Kit

References

1. Tietze, F., (1969) Analytical Chemistry 27, 502-520. 2. Guntherberg, H. and Rost, J., (1966) Analytical Biochemistry 15, 205-210. 3. Griffith, O.W., (1980) Analytical Biochemistry 106, 207-212. 4. Richie, J.P. Jr., et al, (1996) Clinical Chemistry 42, 64-70. 5. Anderson, M., (1996) Glutathione in Free Radicals, A Practical Approach, 213.