Glutathione Peroxidase 1 (GPX1, GPx-1, Cellular Glutathione Peroxidase, HGNC:4553, GSHPX1, GSHPx-1 MGC14399, MGC88245)

Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. It belongs to the glutathione peroxidase family. \n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen and Paraffin): 1:2000- 1:20,000\nWestern Blot: 1:10,000- 1:100,000. Under reducing conditions recognizes a 22kD protein in human brain tissue and glutathione peroxidase isolated from human erythrocytes.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Tissues:\nHuman and rat brain microglia.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8127-11B

Size

100ul

Applications

IHC, WB

Hosts

Sheep

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder. Reconstitute with 100ul of sterile dH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Sukenaga Y, et al. Nucleic Acids Res. 15:7178-7178(1987). ; 2. Ishida K, et al. Nucleic Acids Res. 15:10051-10051(1987). ; 3. Mullenbach G.T, et al. Nucleic Acids Res. 15:5484-5484(1987). ; 4. Chada S, et al. Genomics 6:268-271(1990). ; 5. Moscow J.A, et al. J. Biol. Chem. 267:5949-5958(1992). ; 6. Forsberg L, et al. Hum. Mutat. 13:294-300(1999). ; 7. Kote-Jarai Z, et al. Prostate Cancer Prostatic Dis. 5:189-192(2002). ; 8. Hamanishi T, et al. Diabetes 53:2455-2460(2004). ; 9. Ichimura Y, et al. J. Urol. 172:728-732(2004). ; 10. Strausberg R.L, et al. Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).

Additional Info

Recognizes human glutathione peroxidase 1 (GPx-1) protein. Species Crossreactivity: rat.

Alternative Names

GPX1, GPx-1, Cellular Glutathione Peroxidase, HGNC:4553, GSHPX1, GSHPx-1 MGC14399, MGC88245