Glutathione Peroxidase 4 (GPX4; also known as PHGPx) is a monomeric, 21kD member
of the glutathione peroxidase family of proteins. It is widely expressed and serves to both
protect cell membranes from phospholipid and cholesterol hydroperoxidases, and form an
inactive enzyme structural component of the sperm mitochondrial capsule. Human GPX4 is
197 amino acids (aa) in length and contains a mitochondrial targeting sequence (aa 1-27)
and an enzymatically active selenocysteine at Sec73. During sperm maturation, the
targeting sequence is cleaved, and GPX4 may form inactive covalently-linked oligomers.
There are multiple splice variants. One is ubiquitously expressed, cytoplasmic, and shows
an alternate start site at Met28. A second is 226 aa in length, nuclear, and shows an
Arg-rich 65 aa substitution for aa 1-28. Over amino acids 74-168, human GPX4 shares
93% aa identity with mouse GPX4.
Applications:
Suitable for Western Blot. Other applications not tested.
Storage:
Lyophilized samples are stable for twelve months from date of receipt when stored at -20 degrees C to -70 degrees C. Upon reconstitution, the antibody can be stored at 2 degrees -8 degrees C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquotted and stored frozen at -20 degrees C to -70 degrees C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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