Glutathione Peroxidase BioAssay(TM) Kit

Supplier:

United States Biological

Cat no: G8127-02

Glutathione Peroxidase BioAssay(TM) Kit

Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes play important roles in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. In this Glutathione Peroxidase Activity Assay, GPx reduces Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH (easily measured at 340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5mU/ml of GPx in samples.\n\nKit Components:\nG8127-02A: GPx *Assay Buffer, 1x25ml. Cap Code: WM\nG8127-02B: NADPH (lyophilized), 1x1vial. Cap Code: Blue\nG8127-02C: Glutathione Reductase, 1x1vial. Cap Code: Green\nG8127-02D: Glutathione (GSH; lyophilized), 1x1vial. Cap Code: Brown\nG8127-02E: Cumene Hydroperoxide, 1x1vial. Cap Code: Yellow\nG8127-02F: GPx Positive Control (lyophilized), 1x1vial. Cap Code: Red\n\nStorage and Stability: \nStore the kit at -20 degrees C; protect from light. Allow *Assay Buffer to warm to RT before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8127-02

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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