Glutathione Reductase (Glutathione Reductase, Mitochondrial, GR, GRase, GSR, GLUR, GRD1)

Glutathione reductase (GSR or GR) is a crucial flavoenzyme in the antioxidant defense system that maintains high levels of reduced glutathione in the cytosol. It belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family and is a flavoprotein homodimer of two equal subunits with one mole of noncovalently bound FAD in each subunit. GR reduces glutathione disulphide (GSSG) to the sulfhydryl form (GSH) which is an important cellular oxidant and is used by glutathione peroxidase to detoxify hydrogen peroxide. Apart from NADPH, GR also uses FAD as a cofactor for the process. In sperm, GR and glutathione peroxidase function as antioxidant enzymes and thus may inhibit lipid peroxidation. GR is stimulated by melatonin and is reportedly irreversibly inhibited by a number of oxygen radical generating systems.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Liver lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8127-19D

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Lenzi, A. et al. Hum. Reprod. 9:2044-2050 (1994). 2. Reiter, RJ. et al. Ann. N.Y. Acad. Sci. 854:410-424 (1998). 3. Wan, WY. et al. J. Mol. Biol. 286:1633-1649 (1999).

Additional Info

Recognizes s. cerevisiae GR. Species Crossreactivity: human.