Glutathione-S-Transferase (GST) Control Protein will bind to glutathione affinity resin and will be detected by GST. Affinity tags are appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include Glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
Source:
Glutathione-S-Transferase, purified by chromatography from E.coli preparations.
Applications:
Suitable for use as a control in affinity purification and in Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.