Glutathione S-transferase (GST) Fluorometric BioAssay(TM) Kit

Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. The GST Fluorometric Activity Assay Kit provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (ex/em = 380/461nm) when reacts with glutathione. GST catalizes the MCB-glutathione reactions and the fluorescence levels are proportionally to the amounts of GST presence in the reaction. Thus, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified\nprotein fraction, and also quantitate GST-tagged fusion protein.\n\nKit Components:\nGST Assay Buffer 1x10ml\nGST Sample Buffer 1x25ml\nMCB Substrate 1x200ul\nGlutathione (lyophilized) 1x2vials\nGST Standard (200mU/ul) 1x20ul.\n\nStorage and Stability:\nStore the kit at -20 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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