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Glutathione S Transferase, pi (GSTP1, GSTpi1, GST3, DFN7)

Glutathione S Transferase, pi (GSTP1, GSTpi1, GST3, DFN7)

Cat no: G8135-25J

Supplier: United States Biological
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Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11. Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry (Formalin/paraffin): 1-2ug/ml. Boiling the sections is required Immunocytochemistry (Acetone fixed cells): 0.5-1ug/ml. Antigen retrieval by Pepsin and Trypsin is required. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8135-25J
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 50% glycerol. Reconstitute with 1ml sterile PBS.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Morrow, C. S. et al., Gene 75: 3-11 (1989) 2. Islam M. Q. et al., Hum. Genet. 82: 338-342 (1989) 3. Konohana A et al, J Invest Derm 95:119-126 (1990)
Additional info: Recognizes human GSTpi. Species Crossreactivity: rat. Species Sequence Homology: mouse

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