Glutathione S Transferase, Recombinant Human, His-tag, aa1-224 (GST)

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E. coli source. Recombinant human GST, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nRecombinant corresponding to human GST, his-tagged, expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA\nIIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD\nVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8135-12G

Size

100ug

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH7.4, 10% glycerol.

Purity

~95% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.Hayes JD., et al. (1995). Crit Rev Biochem Mol Biol. 30(6):445-600\n2.Bekett GJ., et al. (1987). J. Clin Biochem Nutr. 2:1-24\n3.Smith DB., et al. (1988). HYPERLINK \"javascript:AL_get(this,%20'jour',%20'Mol%20Biochem%20Parasitol.');\" Mol Biochem Parasitol. 27(2-3):249-56.

Additional Info

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