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Glutathione S Transferase, Schistosoma japonicum (GST)

Glutathione S Transferase, Schistosoma japonicum (GST)

Cat no: G8135-04K

Supplier: United States Biological
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pGEX vectors are frequently utilized in bacterial systems to express polypeptides or entire proteins as fusions with glutathione S-transferase. Using this fusion system, a polypeptide sequence is linked to the C-terminus of the 26 kD GST protein from Schistosoma japonicum. The bacterially expressed GST-fusion proteins are purified by absorption with glutathione-agarose beads. The fusion protein is subsequently eluted from the glutathione agarose with an excess of free glutathione. Antibodies to GST are used to detect pGEX-expressed fusion proteins and as immuno-affinity reagents for purification of recombinant proteins. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation . Other applications not tested. Recommended Dilution: ELISA: 0.05ug/ml Western Blot: 1ug/ml Immunoprecipitation: 1-10ug Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8135-04K
Hosts: Mouse
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as lyophilized powder from 2X PBS, PEG and sucrose, 0.09% sodium azide. Reconstitute with 1ml dH20.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by thiophilic adsorption and size exclusion chromatography.
Additional info: Recognizes Glutathione S Transferase (GST)(pGEX) of Schistosoma japonicum encoded by pGEX expression vectors. Does not interact with human, mouse, rat mammalian GST proteins.

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