Glutathione S Transferase, Schistosoma japonicum, Recombinant (GST)

Glutathione S-Transferase, GST, Glutathione S-transferase class-mu 28kD isozyme, GST 28, EC 2.5.1.18, Sj28GST, Sj28 antigen.\n\nAntioxidant enzyme Glutathione S-Transferase (GST) is thought to do the primary cellular defense mechanism against reactive oxygen species. GST reduces lipid hydroperoxides through its Se-independent glutathione peroxidase activity. The enzyme also detoxifies lipid peroxidation end products such as 4-hydroxynonenal (4-HNE). The soluble GST is a 26kD protein which occurs as a dimer in all aerobic organisms. Each monomer has two domains, one that binds GSH and is an /-structure similar to thioredoxin and the other, all helical, that binds the hydrophobic substrate. The GST -fusion protein expression system is a widely used recombinant protein expression system that allows a peptide or a regulatory protein domain to be expressed as a fusion to the C-terminus of Schistosoma japonicum GST. Fusion proteins also possess GST-enzymatic activity and can undergo dimerization similar to in vivo. The fusion protein can be purified via GST-affinity column chromatography. In most cases, the desired peptides or domains are removed from GST by applying a specific protease that recognizes and cleaves the linker between the protein domain and GST. The technique has been widely used to generate different kinds of proteins for crystallization, molecular immunology studies, the production of vaccines and studies involving protein-protein and protein-DNA interactions.\n\nGST was isolated from an E. coli strain that carries the coding sequence for Schistosoma japonicum GST under the control of a T7 promoter. \n\nUnit Definition: \nA unit is defined as the amount of enzyme that conjuates 1.0umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 degrees C.\n\nAmino Acid Sequence:\nMSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR\n\nApplications:\nSuitable for use in ELISA, Western Blot and Inhibition assays. GST can be used for protein-protein interactions assay and protein-DNA interactions assay. Other applications not tested.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8135-07A

Size

10ug

Form

Supplied as a sterile-filtered liquid in PBS

Purity

(same/more than)90% by SDS-PAGE.

References

1. Glutathione-S-transferase M1, Obesity, Statins, and Autonomic Effects of Particles. Am J Respir Crit Care Med. (2005)172:1529-1533 2. Molecular Cloning and Characterization of a Senescence-Induced Tau-Class Glutathione S-Transferase from Barley Leaves. Plant Cell Physiol. (2005) 46:1540-1548 3. Effect of glutathione-S-transferase polymorphisms on the cancer preventive potential of isothiocyanates: An epidemiological perspective. Mutat Res. (2005) 592:58-67. 4. Glutathione S-transferase M1 gene but not insulin-like growth factor-2 gene or epidermal growth factor gene is associated with prostate cancer. Urol Oncol (2005) 23:225-229. 5. Multi-institutional phase 2 study of TLK286 (TELCYTA, a glutathione S-transferase P1-1 activated glutathione analog prodrug) in patients with platinum and paclitaxel refractory or resistant ovarian cancer. Int J Gynecol Cancer (2005)15:593-600. 6. Suppression of glutathione S-transferase placental form-positive foci development in rat hepatocarcinogenesis by Chlorella pyrenoidosa. Oncol Rep (2005) 14:409-414.