Glutathione (GSH), a ubiquitous low molecular mass thiol, is important for a variety of biological functions including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSH synthetase acts as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamul-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degrees C. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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