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Glyceraldehyde 3 Phosphate Dehydrogenase (GAPD, GAPDH) (Biotin)

Glyceraldehyde 3 Phosphate Dehydrogenase (GAPD, GAPDH) (Biotin)

Cat no: G8140-01E

Supplier: United States Biological
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues , GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntington's and Alzheimer's disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8140-01E
Reactivities: Human, Mouse, Rat, Bovine, Chicken/Bird, Goat, Rabbit
Hosts: Goat
Applications: Western Blot
Conjugates: Biotin
Size: 40ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 30% glycerol. Labeled with Biotin.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes GAPDH. Species Crossreactivity: human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell or tissue lysate

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