Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control (GAPD, GAPDH, G3PD, MGC88685)

Glyceraldehyde-3-phosphate dehydrogenase,G3PDH/GAPD/G3PD (EC 1.2.1.12) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). G3PDH is a cytosolic enzyme. It is thought to be a tetramer of identical chains ~36kD. G3PDH (mouse/rat/rabbit 333; human 335-aa,chromosome 12p13.31-p13.1). The enzyme is present in such widely separated forms as man, lobster, and E. coli. Its rate of evolutionary change is one of the slowest known. \n\nApplications:\nSuitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: Heat once and load 10ul/lane and visualize with G8140-14B. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8140-14A

Size

100ul

Applications

WB

Reactivities

Hum, Mouse, Ch/Bird, Rabbit

Form

Supplied as a liquid in SDS-PAGE sample buffer (reduced)

Purity

Purified

References

1. Tso JY et al (1985) Nucl. Acid Res. 13: 2485-2502. 2.Hanuer A et al (1984) EMBO J. 3: 2627-2633. 3. Arcari P et al (1984) Nucl. Acid. Res. 12: 9179-9189. 4. Meyer-Sigler K et al (1991) PNAS 88: 8460-8464. 5. Tokunaga K et al (1987) Cancer Res. 47: 5616-5619. 6. Allen RW et al (1987) JBC 262: 649-653. 7. Ralser M., et al. (2007).J Biol 21; 6(4):10. 8. Tisdale Ej., et al. (2007).Traffic. 8(6):733-41.