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Glyceraldehyde 3-Phosphate Dehydrogenase, Recombinant, Human (GAPD, GAPDH, G3PD, MGC88685)

Cat no: G8140-14D

Glyceraldehyde 3-Phosphate Dehydrogenase, Recombinant, Human (GAPD, GAPDH, G3PD, MGC88685)

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kD subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. \n\nRecombinant GAPDH protein, 1-335aa, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nMolecular Weight: 36kD (335 aa)\n\nSequence: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD \nSTHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA \nHLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI \nVEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG \nMAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT\nHSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE\n\n\n

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SPECIFICATIONS

Catalog Number

G8140-14D

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris pH 8.0, 1mM EDTA, 1mM DTT, 20% glycerol.

Purity

(same/more than) 95% by SDS-PAGE

References

1. Tso JY et al (1985) Nucl. Acid Res. 13, 2485-2502;\n2.Hanuer A et al (1984) EMBO J. 3, 2627-2633;\n3. Arcari P et al (1984) Nucl. Acid. Res. 12, 9179-9189;\n4. Meyer-Sigler K et al (1991) PNAS 88, 8460-8464;\n5. Tokunaga K et al (1987) Cancer Res. 47, 5616-5619;\n6. Allen RW et al (1987) JBC 262, 649-653\n7. Ralser M., et al. (2007).J Biol 21;6(4):10\n8. Tisdale Ej., et al. (2007).Traffic. 8;(6):733-41

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