Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.
Sample Type:
Animal tissues and cell culture: adherent or suspension cells.
Intended Use:
This kit can be used for measurement of Glycerol-3-Phosphate in various tissues/cells, analysis of carbohydrate/lipid metabolism cell signaling and drug screening.?
Test Principle:
The Glycerol-3-Phosphate BioAssay(TM) kit is a sensitive, fast and easy-to-use kit. In this assay, G3P is oxidized by G3P Enzyme Mix to form an intermediate, which reduces a nearly colorless probe to a colored product with strong absorbance at 450nm. This assay kit can detect G3P less than 2nmol/well and can be used for a variety of sample types.
Kit Components:
G3P Assay Buffer, 1x25ml
G3P Enzyme Mix, lyophilized, 1x vial
G3P Probe, lyophilized, 1x vial
G3P Standard, lyophilized, 1x vial
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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