Glycine Receptor, alpha1 (GLRA1, lycine receptor 48kD subunit, Glycine receptor strychnine-binding subunit, Glycine receptor subunit alpha-1, MGC138878, MGC138879, STHE)

Glycine is an important inhibitory neurotransmitter in the mammalian central nervous system, especially in the brainstem and spinal cord. It acts by binding to anion-conducting glycine receptors that belong to a superfamily of ligand-gated ion channels. Glycine receptors were first purified as strychnine binding sites in membrane fractions of adult spinal cord from rats (1). These strychnine-sensitive binding sites are different from the strychnine-insensitive binding sites found in the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Adult glycine receptors are heterodimers composed of two subunits of 48kD and 58kD, denoted as a1 and b subunits. Fetal glycine receptors are homomers composed of a2 subunits.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1:1000\nImmunohistochemistry (Frozen): 1:1000\nOptimal dilution determined by the researcher. \n\nPositive control: \nCell extracts containing recombinant human glycine receptors and homogenates of rat spinal cord.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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