Glycine Receptor, alpha1 (Glycine Receptor alpha 1, Glycine Receptor Subunit alpha-1, Glra1, Glycine Receptor 48 kD Subunit, Glycine Receptor Strychnine-binding Subunit, Glyr, [a]Z1)

Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been as a model system for identifying binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor a-subunit changes from a2 (fetal) to a1 (adult).\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (frozen): 1:1000\nWestern Blot: 1:1000-2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat brain stem or spinal cord\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8167-01A

Size

50ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder in 5mM ammonium bicarbonate.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Lynch JW. 2004. Molecular structure and function of the glycine receptor chloride channel. Physiol Rev.84:1051-1095. 2. Colquhoun D, Sivilotti LG. 2004. Function and structure in glycine receptors and some of their relatives. Trends Neurosci. 27:337-344. 3. Cascio M. 2004.Structure and function of the glycine receptor and related nicotinicoid receptors. J Biol Chem. 7:19383-19386.

Additional Info

Recognizes rat Glycine Receptor.