Glycine Transporter-2 (GLYT2)

Glycine is one of the major inhibitory neurotransmitters in the spinal cord and brain stem. Glycine acts on the strychnine-sensitive glycine receptors, a pentameric Cl-channel, to generate inhibitory postsynaptic potentials. Glycine can modulate excitatory neurotransmission as an obligatory co-agonist with glutamate at NMDA-activated glutamate receptors via binding site on the NMDA receptor, which is distinct from the strychnine-sensitive glycine receptor-binding site. Glycinergic synapses are primarily present throughout the spinal cord, brain stem and cerebellum, as well as in the retina and a few other brain regions. Neurotransmitter action is terminated by re-uptake of glycine via specific high affinity transporter protein located at the plasma membrane of neuron and glial cells. The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms (GLYT1 and GLYT2). Form 1 has three other known isoform (GLYT1a, GLYT1b and GLYT1c) which may be produced by alternative spicing or promoter usage. Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and expressed from the same gene.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL).\nELISA: 1:10,000-1:50,000. Coat with 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nG8168-25: Glycine Transporter-2, Rat, Control Peptide (GLYT2)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8168-21

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. Liu, Q.R., et al., JBC 268: 22802 (1993). 2. Jursky, F., et al., J. Neurochem. 67: 336(1996). 3. Liu, Q.R., Nelson, H., Mandiyan, S., Lopez-Corcuera, B., Nelson, N.: Cloning and expression of a glycine transporter from mouse brain. FEBS Letts. 305: 110-114 (1992). 4. Borowsky, B., Mezey, E., Hoffman, B.J.: Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene. Neuron 10: 851-863 (1993). 5. Kim, K.M., Kingsmore, S.F., Han, H., Yang-Feng, T.L., Godinot, N., Seldin, M.F., Caron, M.G., Giros, B.: Cloning of the human glycine transporter type 1: molecular and pharmacological characterization of novel isoform variants and chromosomal localization of the gene in the human and mouse genomes. Mol. Pharmacol. 45: 608-617 (1994). 6. Adams, R.H., Sato, K., Shimada, S., Tohyama, M., Puschel, A.W., Betz, H.: Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents. J. Neurosci. 15: 2524-2532 (1995). 7. Evans, J., Herdon, H., Cairns, W., O'Brien, E., Chapman, C., Terrett, J., Gloger, I.: Cloning, functional characterisation and population analysis of a variant form of the human glycine type 2 transporter. FEBS Letts. 463: 301-306 (1999). 8. Horiuchi, M., Nicke, A., Gomeza, J., Ashcrafi, A., Schmalzing, G., Betz, H.: Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopusoocytes. PNAS USA 98: 1448-1453 (2001).

Additional Info

Recognizes rat GLYT2. No homology is seen with either GLYT1 or other transporters. Species Sequence Homology: mouse, and human: 100%