Glycine Transporter-2 (GLYT2) (Glycerol Free)

The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms-GLYT1 and GLYT2. Form 1 has three other known isoforms (GLYT1a, GLYT1b, and GLYT1c) which may be produced by alternative splicing or promoter usage. Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and are expressed from the same gene. Although the N-terminal part of mouse and rat GLYT1a is identical in mouse and rat, the N-terminus of GLYT1b is significantly different in these two species. The GLYT1c subtype has only been reported in humans. GLYT1a is expressed in CNS and peripheral organs. GLYT1b is localized in the CNS. Both neuron and Glial cells have GLYT1. More recent studies indicate that GLYt1b may not be brain specific. Immunolocalization studies on GLYT2 suggest that GLYT2 is responsible for terminating the neurotransmission at the strychnine synapses.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 1:10,000-1:50,000 using 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: G8168-25\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8168-23

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide. Glycerol free.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Liu, Q.R., et al., JBC 268: 22802 (1993). 2. Jursky, F., et al., J. Neurochem. 67: 336(1996). 3. Liu, Q.R., Nelson, H., Mandiyan, S., Lopez-Corcuera, B., Nelson, N.: Cloning and expression of a glycine transporter from mouse brain. FEBS Letts. 305: 110-114 (1992). 4. Borowsky, B., Mezey, E., Hoffman, B.J.: Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene. Neuron 10: 851-863 (1993). 5. Kim, K.M., Kingsmore, S.F., Han, H., Yang-Feng, T.L., Godinot, N., Seldin, M.F., Caron, M.G., Giros, B.: Cloning of the human glycine transporter type 1: molecular and pharmacological characterization of novel isoform variants and chromosomal localization of the gene in the human and mouse genomes. Mol. Pharmacol. 45: 608-617 (1994). 6. Adams, R.H., Sato, K., Shimada, S., Tohyama, M., Puschel, A.W., Betz, H.: Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents. J. Neurosci. 15: 2524-2532 (1995). 7. Evans, J., Herdon, H., Cairns, W., O'Brien, E., Chapman, C., Terrett, J., Gloger, I.: Cloning, functional characterisation and population analysis of a variant form of the human glycine type 2 transporter. FEBS Letts. 463: 301-306 (1999). 8. Horiuchi, M., Nicke, A., Gomeza, J., Ashcrafi, A., Schmalzing, G., Betz, H.: Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopusoocytes. PNAS USA 98: 1448-1453 (2001).

Additional Info

Recognizes rat GLYT2. No homology is seen with either GLYT1 or other transporters. Species Sequence Homology: Human and mouse.